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. 2011 Jul;31(14):2920–2933. doi: 10.1128/MCB.05431-11

Fig. 8.

Fig. 8.

Trilaminar cell layer and markers of cell adhesion and cell polarity in Llgl2−/− and Llgl2+/− placentas. (A and B) Schematic representation of mouse placenta (A) and trilaminar cell layer separating maternal and embryonic blood spaces (B). (C to E) Pseudocolored electron micrographs from the labyrinth layers of E11.5 Llgl2−/− and Llgl2+/− placentas. The three layered interhemal barrier at the maternofetal interface indicates the maternal (enucleated) and fetal blood cells (nucleated) separated by the endothelium (E) that lines fetal lumen and two syncytiotrophoblast layers (ST-I and ST-II). Mononuclear sinusoidal giant trophoblast cells (Sgtc) are seen associated with the ST-I cell layer (scale bar, 2 μm). Images are shown at low (C), intermediate (D), and high (E) magnifications. Arrows in panel E show small areas of membrane separation between ST-I and ST-II cell layers in Llgl2−/− placentas. The scale bar in panel C corresponds to 2 μm in panel C, 1 μm in panel D, and 0.5 μm in panel E. (F) Immunohistochemical stainings of sagittal placental sections through the central region of E13.5 Llgl2−/− and Llgl2+/− placentas with anti-E-cadherin, anti-β-catenin, anti-atypical PKC, and anti-connexin 26 antibodies (22). Blue is hematoxylin counterstain. (G and H) Western blot analysis of total protein extracts from E11.5 (G) and E13.5 (H) Llgl2−/− and Llgl2+/− placentas with anti-E-cadherin (E-cad), anti-β-catenin (β-cat), anti-atypical PKC zeta (aPKC), and anti-β-actin antibodies.