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. 2011 Jul;31(14):2947–2959. doi: 10.1128/MCB.05589-11

Fig. 2.

Fig. 2.

eIF4G is a substrate for PKCα. (A) Co-IP of PKC isoforms with eIF4G from Tet-induced, TPA-stimulated HEK293eIF4G-b cells. Serum-starved HEK293eIF4G-b cells were Tet induced for 24 h and treated for 60 min with DMSO (0) or 200 nM TPA. Cell lysates were subjected to Flag-IP followed by immunoblotting with the indicated antibodies. The experiment was repeated four times with similar results. A representative assay is shown. (B) Immunoblot of eIF4G and its cleavage products with site-specific antibodies. Serum-starved HEK293eIF4G-b cells were Tet or mock induced for 24 h and treated for 15 min with DMSO (0) or 200 nM TPA. The experiment was repeated twice with similar results. A representative assay is shown. M, molecular mass.