Fig. 5.
Hepatocyte Snail1 is required for efficient liver fibrogenesis. (A) Wild-type or CKO mice were treated with CCl4 for 2 weeks, at which time liver tissue was isolated. The tissue was stained with Sirius red (left two columns; scale bar = 200 μm) or immunostained for collagen I (green; middle column), collagen III (red; fourth column from left), or FSP1 (encoded by the S100A4 gene) (green; right column) (scale bars = 50 μm) with TOTO-3 counterstaining. (B) Induction of collagen I and III deposition versus uninjured liver was quantified by morphometric analysis (n = 6; *, P < 0.05). The error bars indicate SEM. (C) Scatter plot demonstrating distribution of fibrosis scores for each genotype (*, P = 0.003). Horizontal lines denote mean fibrosis scores.