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. 2011 Jun;31(12):2528–2542. doi: 10.1128/MCB.01285-10

Fig. 4.

Fig. 4.

Neurospora centromeres are heterochromatic. (A) LG IV is shown as a representative chromosome to compare CenH3-GFP localization (red, NMF166) to modified histones. H3K4me2 (black; NMF229) and H3K4me3 (black; NMF39) are enriched along chromosome arms in genes but absent from the centromere. The heterochromatic mark H3K9me3 (black, NMF166) is found at both dispersed heterochromatin along the chromosome arms and overlapping CenH3 at the centromere. DNA methylation (5meC, black; NMF39) is found at dispersed heterochromatin and the outer edges of the centromere but not the centromere core. (B and C) Expanded view of ChIP-seq read counts for chromatin modifications at Cen II (left) and Cen IV (right) compared to CenH3 enrichment (NMF166). (B) The euchromatic marks, H3K4me2 (NMF229) and H3K4me3 (NMF39) are absent from CenH3-enriched regions. (C) The heterochromatic mark, H3K9me3 (NMF39) is found throughout the CenH3-enriched region and in adjacent pericentric heterochromatin, but DNA methylation (5mC; NMF39) is largely restricted to pericentric heterochromatin (at the centromere edges). Scales and abbreviations are as shown in Fig. 2.