Fig. 6.

CenH3 mislocalization in heterochromatin mutants. (A) ChIP-PCRs of centromere repeats compared to the hH4-1 euchromatic control show that distribution of CenH3-GFP is changed in both the dim-5 (NMF238) and hpo (NMF232) mutants, especially at repeats in the centromere periphery. ChIPs were repeated at least two times, and PCRs were performed at least two times with DNA from each replicate ChIP. Results from one representative experiment are shown. (B) Enrichment of CenH3 at centromere repeats relative to hH4-1 was calculated and plotted for the wild-type (WT), dim-5, and hpo strains. Variability in the amount of background (hH4-1 amplification) made quantification of enrichment difficult in some experiments.