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CenH3 localization is changed in heterochromatin mutants. ChIP-seq of CenH3-GFP was performed on dim-5 (NMF239) (A) or hpo (NMF232) (B) mutants and revealed altered patterns of CenH3 enrichment compared to that of the WT (NMF166) and thus CenH3 localization on most chromosomes.