Fig. 8.

Occupancy of CenH3 is confined to smaller regions on most centromeres in heterochromatin mutants. Centromere boundaries were determined as the outermost 1-kb windows with enrichment significantly above background levels at a cutoff of P ≤ 0.0001. Centromere sizes are plotted here as a bar graph, and approximate sizes of centromeric regions for WT (NMF166), dim-5 (NMF239), and hpo (NMF232) strains are noted on the right. In some cases (e.g., Cen I and Cen III in dim-5 strains), single windows with significant CenH3-GFP enrichment were found separated by several kilobases from the “main” centromeric DNA.