Fig. 9.
The dim-5 mutant and to a lesser extent the hpo mutant have defects that cause slow-growth phenotypes. (A) Linear growth of WT (NMF39) and CenH3-GFP-tagged strains (A, NMF168; B, NMF169). (B) Linear growth of dim-5 (N2264) and dim-5 CenH3-GFP strains (A and B, NMF238; C, NMF239; D and E, NMF240). (C) Linear growth of hpo (N2552) and hpo CenH3-GFP (NMF232) strains in race tubes at 25C. WT and CenH3-GFP-tagged strains have similar growth rates. The hpo mutants generally have lower growth rates. We recovered only a few hpo CenH3-GFP strains, which grow better than the original hpo mutants. The dim-5 mutation causes decreased but variable growth rates, which can be exacerbated by the presence of the CenH3-GFP allele.
