Table 4.
Regions with centromeric DNA on the seven Neurospora chromosomes
| LG | Cen posa (kb) | Cen size, kb (%)b | Positions of pericentric regionsc (kb) | No. of ctd | First gene left arm | First gene right arm | Predicted gene in centromeree |
|---|---|---|---|---|---|---|---|
| I | 3736–3969 | 233.4 (2.4) | 3735–3736 | 4 | 3732488–3734801 | 3972683–3974681 | 3819644–3819946 |
| 3969–3980 | (NCU08579) | (NCU09892) | (NCU08585) | ||||
| II | 1105–1346 | 240.8 (5.4) | 1090–1105 | 6 | 1091322–1092875 | 1366146–1367085 | 1152277–1154521 |
| 1346–1366 | (NCU03328) | (NCU05338) | (NCU11930) | ||||
| III | 705–951 | 246.0 (4.7) | 680–705 | 4 | 681888–682855 | 965755–967346 | 813119–139778 |
| 951–970 | (NCU10596) | (NCU07815) | (NCU05742) | ||||
| IV | 894–1068 | 174.0 (2.9) | 870–894 | 1 | 872242–874450 | 1088600–1089972 | 994038–994787 |
| 1068–1090 | (NCU04963) | (NCU04983) | (NCU04974) | ||||
| V | 932–1209 | 276.8 (4.3) | 890–932 | 5 | 897183–897949 | 1219940–1221421 | None |
| 1209–1230 | (NCU09667) | (NCU11600) | |||||
| VI | 2811–3060 | 249.0 (5.9) | 2770–2811 | 11 | 2766824–2769514 | 3080312–3087794 | None |
| 3060–3080 | (NCU03846) | (NCU12161) | |||||
| VII | 1801–2089 | 287.4 (6.8) | 1770–1801 | 1 | 1770246–1772345 | 2120198–2122450 | None |
| 2090–2120 | (NCU02438) | (NCU11384) |
Position of centromeric (Cen) region on linkage group (LG) in kb.
Size of centromeric (Cen) region in kb and percentage of chromosome that is centromeric DNA, which is large compared to that for S. pombe (0.6 to 4.5%).
Positions of pericentric regions, defined as high in 5-methylcytosine and H3K9me3 enrichment but having little CenH3 enrichment, typically ∼19 to 35 kb of AT-rich DNA on either side of each centromere.
Number of contigs (ct) in the centromeric regions.
The gene within Cen II (NCU11899) is on a short ∼2-kb segment and likely misassembled with respect to the neighboring contigs. All other hypothetical or predicted genes annotated within the centromeres of LG I, III, and IV have no known transcripts, are not associated with H3K4me2 or H3K4me3, and are enriched for CenH3, CEN-C, and CEN-T and likely pseudogenes.