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. 2011 Jun;31(12):2439–2452. doi: 10.1128/MCB.01246-10

Fig. 5.

Fig. 5.

Endogenous PR target gene expression is attenuated in cells containing S79/81A PR relative to wt PR. (A, B, and C) Top: T47D-Y cells stably expressing either wt PR-B or S79/81A PR, or unmodified (PR-null) cells, were starved for 18 h in serum-free medium, followed by treatment with 10 nM R5020 or EtOH for 6 h. BIRC3 (A), HSD11β2 (B), HbEGF (C), or β-actin (internal control) mRNA levels were analyzed by qPCR. Middle: T47D-Y cells stably expressing wt PR-A, PR-B, or S79/81A PR were serum starved for 18 h, followed by treatment with 10 nM R5020 or EtOH for 6 h. BIRC3 (A), HSD11β2 (B), HbEGF (C), or 18S (internal control) mRNA levels were analyzed by qPCR. Asterisks indicate statistical significance (P < 0.05; determined using an unpaired Student's t test) compared to the respective treatment group (EtOH or R5020) in control cells (PR-null or PR-A). Bottom (C): T47D-YB cells were starved for 18 h in serum-free medium. Cells were then pretreated with TBB (10 μM) or DMSO (vehicle) for 30 min, followed by 60 min of 10 nM R5020. HbEGF and β-actin (internal control) mRNA expression was analyzed using qPCR. Error bars represent means ± SD of triplicate measurements.