Figure 4.

The Na⁺-K⁺ pump blocker strophanthidin blocks both the firing-induced and basal channel opening. A, B, Channel activity was recorded first in ACSF, then in strophanthidin; histograms are shown for a representative experiment (A) and for the ensemble average (B, n = 13). Experiments were performed and analyzed with the experimenters blind to the identity of the washed-in solution. C, The effects of strophanthidin (or 0.1% DMSO) are summarized (as in Fig. 3B). For strophanthidin (Stroph), the fitted slopes were (peak) −0.71 ± 0.15 and (full) −0.75 ± 0.14, consistent with ∼70–75% inhibition. For DMSO, the fitted slopes were (peak) 0.96 ± 0.06 and (full) 1.35 ± 0.31 (n = 11).