Table 10.
Primers and probes for real-time PCR detection of virulence genes developed for this study
| Target genea | Forward primer, reverse primer and probe sequences (5'-3') | Location within sequences | Gene Bank accession no. |
|---|---|---|---|
| nleG6-2 (Z2150) | ATATGCTCTCTATATGATAAGGATG | 1928877-1928901 | AE005174 |
| AAAGTGACATTCGTCTTTTCTCATA | 1928996-1928872 | ||
| [6FAM]CGTTAGTGCAACTTGTTGAAACTGGTGGAA[BHQ1] | 1928902-1928931 | ||
| nleG5-2 (Z2151) | AGACTATTCGTGGAGAAGCTCAAG | 1929199-1929222 | AE005174 |
| TATTGAAGGCCAATCTGGATG | 1929337-1929317 | ||
| [6FAM]TGGATATTTTATGGGAAGTCTTAACCAGGATGG[BHQ1] | 1929269-1929301 | ||
| espK | ATTGTAACTGATGTTATTTCGTTTGG | 1673295-1673320 | AE005174 |
| GRCATCAAAAGCGAAATCACACC | 1673419-1673397 | ||
| [6FAM]CAGATACTCAATATCACAATCTTTGATATATAAACGACC[BHQ1] | 1673330-1673368 | ||
| bfpA | CCAGTCTGCGTCTGATTCCA | 2756-2775 | FM180569 |
| CGTTGCGCTCATTACTTCTGAA | 2816-2795 | ||
| TAAGTCGCAGAATGC-MGB | 2777-2791 |
a) Z2150 and Z2151 derive from OI-57 [24]