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. 2011 Jul 11;6(7):e21662. doi: 10.1371/journal.pone.0021662

Figure 4. Cdkn1a DNA methylation levels are dynamically regulated in early postnatal livers.

Figure 4

(A) UCSC browser schematic of the Cdkn1a region analysed using SEQUENOM. Amplicons (1 and 2), individual CpG dinucleotides (CpG sites) as well as the 19 analysable T-cleavage-derived CpG units (SEQUENOM fragments) are represented as custom tracks. (B) Relative difference in DNA methylation (MHF compared to control) in liver at 19 CpG units across the Cdkn1a CpG Island (UCSC position chr20:7384350–7384590) at postnatal day 2 and at postnatal day 27. (C) Average absolute DNA methylation levels in liver across the entire CpG Island in CTRL and MHF offspring at postnatal day 2 and postnatal day 27. (D) Absolute DNA methylation at specific CpG units shown to be significantly differentially methylated (positions 7384957 and 7384643) in CTRL and MHF postnatal day 2 livers. P2, postnatal day 2; P27, postnatal day 27; CTRL, control offspring; MHF, maternal high fat offspring.