Activation of other human TRP channels by DEP. Over-expressing (TRPA1, M8, V2, V3, and V4), transiently transfected (TRPV1), and untransfected HEK-293 cells were treated with DEP (0.77 mg/ml in-well concentration) or a solution of a prototype TRP channel agonist at a concentration that yielded the maximum response relative to control HEK-293 cells. Agonists were TRPA1 – 150 µM AITC; TRPM8 – 20 µM icilin; TRPV1 – 20 µM nonivamide; TRPV2 – 100 µM Δ9-tetrahydrocannabinol TRPV3 – 300 µM carvacrol; TRPV4 – 12.5 nM GSK 1016790A. Data are the percent maximum response elicited by ionomycin (10 µM) with the vehicle control subtracted. N.D. = no response detected. *Indicates p<0.05 using ANOVA with Bonferroni correction (n=3).