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. Author manuscript; available in PMC: 2012 Jun 20.
Published in final edited form as: Chem Res Toxicol. 2011 May 25;24(6):950–959. doi: 10.1021/tx200123z

Figure 5.

Figure 5

Mutation of C621, C641, and C665 to A and lysine K710 to A of TRPA1 (the TRPA1–3CK mutant) inhibits DEP-induced calcium flux. HEK-293 cells were transiently transfected with human TRPA1 or the TRPA1–3CK mutant and treated with 150 µM AITC, suspensions of DEP or CFA1 to a final in-well concentration of 0.77 mg/mL, or an equivalent concentration of DEP-EtOH extract. Data are the percent maximum response elicited by ionomycin (10 µM) with the vehicle control subtracted. N.D. = no response detected. *Indicates significant (p<0.05) calcium influx compared to HEK-293 controls, and # indicates a significant reduction in TRPA1–3CK mutant transfected cells compared to wild-type TRPA1 using ANOVA with Bonferroni correction.