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. 2011 Jul 11;6(7):e22118. doi: 10.1371/journal.pone.0022118

Figure 5. Galiellalactone induces apoptosis and inhibits proliferation of ALDH+ prostate cancer cells.

Figure 5

A. ALDH+ and ALDH− cells sorted from DU145 and LNCaP-IL6 cells were treated with 25 µM galiellalactone for 24 h. Apoptotic cells were detected by c-PARP staining. B. Quantification of c-PARP stained apoptotic cells in ALDH+ and ALDH− cells sorted from DU145 and LNCaP-IL6 cells and treated with 25 µM galiellalactone for 24 h. Treatment with galiellalactone significantly increased the amount of c-PARP expressing cells in ALDH+ DU145, ALDH+ LNCaP-IL6 and ALDH− LNCaP-IL6 cells compared to untreated controls (p = 0.019, 0.035 and 0.009 respectively; n = 2). The difference in apoptotic response between galiellalactone treated ALDH+ and ALDH− cells from DU145 and LNCaP-IL6 cells was not significant (p = 0.059 and p = 0.119, respectively; n = 2). C. Galiellalactone decreased the viability of ALDH+ and ALDH− cells sorted from DU145 and LNCaP-IL6 cells. The ALDH+ DU145 cells were significantly more sensitive to galiellalactone compared to the corresponding ALDH− cells at 5 µM (p = 0.0017), 10 µM (p = 0.0010), 25 µM (p = 0.0019) and 50 µM (p = 0.0025). Results are presented as mean per cent of untreated control (n = 2).