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SDS polyacrylamide gel electrophoresis of limited trypsin digestion of PhoP. The lane labeled “M” is the molecular weight marker. Lane 1 is PhoP before trypsin digestion, and lane 2 is after digestion. Calculated molecular weight for PhoP is 27.8 kDa. Protein samples were resolved by SDS-PAGE, and visualized by staining with SimplyBlue. Two bands marked with arrows are sequenced by Edman degradation to identify trypsin cleavage sites.
