Table 2. Sequence of primers used in this study.
Gene | Orientation* | Sequence (5′–3′) | Fragment size (bp) | Reference |
sxt1 | F | CTGGATTTAATGTCGCATAGTG | 150 | Guion et al. (2008) |
R | AGAACGCCCACTGAGATCATC | |||
stx2 | F | GGCACTGTCTGAAACTGCTCC | 255 | Guion et al. (2008) |
R | TCGCCAGTTATCTGACATTCTG | |||
eae | F | ATGCTTAGTGCTGGTTTAGG | 248 | Guion et al. (2008) |
R | GCCTTCATCATTTCGCTTTC | |||
ehxA | F | GGTGCAGCAGAAAAAGTTGTAG | 1551 | Schmidt et al. (1995) |
R | TCTCGCCTGATAGTGTTTGGTA | |||
astA | F | CCATCAACACAG TAT ATCCGA | 111 | Yamamoto & Echeverria (1996) |
R | GGTCGCGAGTGACGGCTTTGT | |||
chuA | F | GACGAACCA ACGGTCAGGAT | 279 | Clermont et al. (2000) |
R | TGCCGCCAGTACCAAAGACA | |||
yjaA | F | TGAAGTGTCAGGAGACGCTG | 211 | Clermont et al. (2000) |
R | ATGGAGAATGCGTTCCTCAAC | |||
tspE | F | GAGTAATGTCGGGGCATTCA | 152 | Clermont et al. (2000) |
R | CGCGCCAACAAAGTATTACG |
F, Forward primer; R, reverse primer.