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. Author manuscript; available in PMC: 2011 Dec 15.
Published in final edited form as: J Immunol. 2010 Nov 15;185(12):7146–7150. doi: 10.4049/jimmunol.1002163

Figure 1.

Figure 1

Surface expression of PD-L2 (CD273), CD80, and CD73 define phenotypic subsets of IgM and isotype-switched memory B cells. Memory cells were generated after adoptive transfer of Vh186.2 Sd-Tg BALB/c splenic B cells into AM14 × Vκ8R Tg CB.17 recipients, immunization with NP-CGG precipitated in alum, and 12–20 wk rest. Naive cells were harvested from unimmunized Vh186.2 Sd-Tg mice. The percentage of the parent population of each quadrant or gate is indicated for individual mice shown. A, FACS plots of splenocytes stained with Abs to the indicated markers gated on live, NIP-binding κneg splenic B cells. Representative plots for >11 mice are shown. B, Representative graphs of IgM staining of memory B cell subpopulations defined as in A. Individual quadrants are depicted clockwise, beginning with the upper right. Parallel stains with anti-IgG1 (data not shown) confirmed the IgM− gates. Naive B cells were lacking IgM− and IgG1+ populations (data not shown). Representative plots from at least five individual mice per dose are shown. Specific memory subpopulations discussed in the text are highlighted in color.