Table 1.
Shield | 80% epiboly | 5-8 somite | 24 hpf | 48 hpf | 50–56 hpf | 70 hpf | 96 hpf | 120 hpf | |
---|---|---|---|---|---|---|---|---|---|
Cyr61-c5 | − | − | − | + | + | + | + | − | − |
Cyr61-c8 | − | − | + | − | − | − | − | − | − |
Cyr61-c23 | − | + | + | + | + | − | + | + | + |
CTGF-c19 | − | − | + | + | + | + | + | + | + |
CTGF-c20 | − | − | + | + | + | + | + | + | + |
WISP1-c16 | − | − | − | − | − | + | − | − | − |
WISP1-c19 | − | − | − | − | − | − | − | − | − |
WISP2-c23 | − | − | − | − | − | − | + | − | − |
WISP3-c20 | − | − | + | + | + | + | + | + | + |
More than 20 embryos were examined for all antisense probes, except for 50–56 hpf; at this time point only WISP1-c16 was assayed in more than 20 embryos. Control hybridizations used sense probes for each zCCN and gave no signal.