Table 2.

Gene Specific Primers Used for 5′RACE of Zebrafish CCN cDNA

zCCN	Gene specific primer for 5′RACE	bp of sequence upstream of starting ATG, obtained by 5′ RACE	Is there an in-frame stop codon upstream of starting Methionine (in 5′RACE sequence)?
zCyr61-c5	5′ TGATACCTGGAGGACACGAGGGGGGTGCGG	162 bp	Yes
zCyr61-c23	5′ GGCATCCGGAGCACAGGAGCACACCGC	114 bp	No
zCTGF-c19	5′ AGGGCTCTCCTGCCTGTCGTGCGCACAC	84 bp	Yes
zCTGF-c20	5′ GGGCGGCACTTCAGGGCAGTGGCATTGTCC	217 bp	Yes
zWISP1-c16	5′ ACCCCATGTGGGCAAACAAGGGCGCTCTTGG	188 bp	Yes
zWISP1-c19	5′ GCAGACTGACACCTGGCGGGCACATGGGA	188 bp	Yes
zWISP2-c23	5′ GGCAGCACTGGCACCCGTCGAGAATGAGAGGAC	267 bp	Yes