Table 3.

Polymerase Chain Reaction Primer Pairs Used to Generate zCCN In Situ Hybridization Probes

zCCN	Forward primer	Reverse primer	Amplicon size
zCyr61-c5	5′CCTACTGAACCCTGCGACCACATTAAA	5′CGAGTAGTGGAACATCCAGCAAAACTG	710 bp
zCyr61-c8	5′GCCTAAACACACACCACAGTACAACC	5′GAAGATCGTCCTCTCTTAGCTCTGC	534 bp
zCyr61-c23	5′GGTGTGTGCCAGACAGCTGAAC	5′CGGCGTCACAGACTTCTTGGT	703 bp
zCTGF-c19	5′GTGTTAGACGGCTGTAGCTGCTGTAA	5′GGCAATTGTGATGACAGGAGCAGGTC	853 bp
zCTGF-c20	5′TCACCTGGTGTAAGCCTAGTTCTGG	5′GGCATGCGCAGGTCTTGATGAAC	847 bp
zWISP1-c16	5′CTGTGACTACAGCGCTGACAAGC	5′GCTGACACAGCCTGAGATGGTGA	568 bp
zWISP1-c19	5′TCAGGGAGGCTTGCAATGAGAAAG	5′GTGAAGTTTTGAGGCTTCTCTGCTCTG	604 bp
zWISP2-c23	5′GTGTGACTACAGCGCCAGCTTT	5′CCTGTAGCTGGGTTGACATTTCCTTC	550 bp