Skip to main content
. Author manuscript; available in PMC: 2011 Jul 12.
Published in final edited form as: J Neurosci Res. 2010 Feb 15;88(3):589–604. doi: 10.1002/jnr.22236

Figure 2. OCRL-1 is expressed in oligodendrocytes and HOG cells.

Figure 2

A) RT-PCR analysis of the expression of OCRL-1 in rat oligodendrocytes and HOG cells. RT reaction was performed using 10 μg of total RNA and primers targeting the C terminus of OCRL-1 (rat and human). PCR reactions were carried out using the cDNA obtained in the RT reaction and primers targeting the C- and the N- termini (lanes 1 and 2). To rule out DNA contamination, PCR was carried out using as templates total RNA (lane 3 and 4). PCR products were separated by electrophoresis on 1 % agarose gels. Oligodendrocytes, lanes 1 and 3; HOG cells, lanes 2 and 4. B) Western blot analysis of OCRL-1 expression in differentiated oligodendrocyte cultures and HOG cells with monoclonal antibody against OCRL-1. Lysate samples containing 20 μg of protein (Oligodendrocytes, lanes 1 and 3; HOG cells, lanes 2 and 4) were resolved by SDS-PAGE on 6 % gels. Proteins were transferred to nitrocellulose membranes by electroblotting. Nitrocellulose membranes were overlaid with media containing monoclonal antibody against OCRL-1 (lanes 1 and 2). Negative control consisted in membranes incubated in the absence of antibody against OCRL-1 (lanes 3 and 4). Bound antibodies were detected by enhanced chemiluminescence.