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. Author manuscript; available in PMC: 2011 Jul 12.
Published in final edited form as: J Neurosci Res. 2010 Feb 15;88(3):589–604. doi: 10.1002/jnr.22236

Figure 3. Coimmunoprecipitation of Rab31 and OCRL-1.

Figure 3

A) Immune complexes formed by treatment of oligodendrocyte lysates with rabbit IgG against Rab31. B) Immune complexes formed by treatment of the lysates with non specific rabbit IgG. Samples were resolved by SDS-PAGE on 10 % gels. Proteins were transferred from gel to nitrocellulose membranes by electroblotting. Nitrocellulose membranes were overlaid with media: containing rabbit IgG against Rab31 (lane 1) or mouse monoclonal anti- OCRL-1 (lane 2). Bound antibodies were detected by enhanced chemiluminescence using goat anti-rabbit antibodies (lane 1) or rabbit antimouse antibodies (lane 2) conjugated to horseradish peroxidase.