Fig. 3.
Salvador deletion potentiates canonical Wnt signaling. Heat map (A) and qRT-PCR validation (B) showing relative transcript levels of Wnt/β-catenin target genes. Values were determined as a mean of three samples ± SD with glyceraldehyde-3-phosphate dehydrogenase control. (C) qRT-PCR of Wnt/β-catenin target genes in β-catenin CKO hearts. (D and E) IF images with quantification (F) of E12.5 heart sections: stain with TO-PRO-3, blue, and α-β-catenin, green. Nuclear β-catenin identified by β-catenin/TO-PRO-3 signal overlap (arrows). Control genotype is Nkx2.5cre; Salvf/+.