Fig. 3.





Levels of ceramides during chronological aging. S. cerevisiae BY4741, isc1Δ and lcb4Δ cells were grown in YPD medium to the post-diauxic phase, washed twice with H2O and kept in H2O at 26°C. (A) total dihydroceramide (dh-Cer); (B) dihydro-C26-ceramide (dh-C26-Cer); (C) total phytoceramide (phyto-Cer); (D) phyto-C26-ceramide (phyto-C26-Cer); (E) total α–hydroxylated-phytoceramide (αHO-phyto-Cer). Data are expressed as pmol of lipid/nmol lipid Pi and are means ± SD of three independent experiments. See Tables S1–S3 for levels of individual ceramide species.