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. Author manuscript; available in PMC: 2012 Jul 1.

Published in final edited form as: Mol Microbiol. 2011 Jun 28;81(2):515–527. doi: 10.1111/j.1365-2958.2011.07714.x

Fig. 4 — SIT4 disruption suppresses the shortened chronological lifespan of isc1Δ cells. S. cerevisiae BY4741, iscΔ, sit4Δ and sit4Δisc1Δ mutant cells were grown in YPD medium to post-diauxic phase. (A) Cells were washed twice with H₂O, and kept in H₂O at 26°C. The viability was determined by standard dilution plate counts and expressed as the percentage of the colony-forming units at time 0h. (B) Protein carbonylation. Protein extracts were derivatized with DNPH and slot-blotted into a PVDF membrane. Immunodetection was performed using an anti-DNP antibody, as described in Materials and Methods. Quantitative analysis of total protein carbonyl content was performed by densitometry using data taken from the same membrane. Data are expressed as the carbonyl content ratio between cells of day 14 and cells of day 0. Values are means ± SD of three independent experiments. *p<0.05.

Fig. 4 — SIT4 disruption suppresses the shortened chronological lifespan of isc1Δ cells. S. cerevisiae BY4741, iscΔ, sit4Δ and sit4Δisc1Δ mutant cells were grown in YPD medium to post-diauxic phase. (A) Cells were washed twice with H₂O, and kept in H₂O at 26°C. The viability was determined by standard dilution plate counts and expressed as the percentage of the colony-forming units at time 0h. (B) Protein carbonylation. Protein extracts were derivatized with DNPH and slot-blotted into a PVDF membrane. Immunodetection was performed using an anti-DNP antibody, as described in Materials and Methods. Quantitative analysis of total protein carbonyl content was performed by densitometry using data taken from the same membrane. Data are expressed as the carbonyl content ratio between cells of day 14 and cells of day 0. Values are means ± SD of three independent experiments. *p<0.05.