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. Author manuscript; available in PMC: 2012 Jul 1.

Published in final edited form as: Mol Microbiol. 2011 Jun 28;81(2):515–527. doi: 10.1111/j.1365-2958.2011.07714.x

Fig. 7 — SIT4 disruption suppresses catalase A deficiency seen in isc1Δ cells and CTA1 overexpression partially suppresses the shortened chronological lifespan of isc1Δ cells. S. cerevisiae BY4741, isc1Δ, sit4Δ and sit4Δisc1Δ mutant cells were grown in YPD medium to post-diauxic phase (PDS). Catalaseactivity was determined (A) spectrophotometrically or (B) detected in situ after non-denaturing polyacrylamide gel electrophoresis, using the H₂O₂/peroxidase system. (C) S. cerevisiae BY4741 and isc1Δ mutant cells transformed with pPGK-M28-I (empty vector) or pCTA1-GFP were grown in SC medium lacking uracil to post-diauxic phase. Cells were washed twice with H₂O, and kept in H₂O at 26°C. The viability was determined by standard dilution plate counts and expressed as the percentage of the colony-forming units at time 0h. (D) CTA1 overexpression increases Cta1p activity in both S. cerevisiae BY4741 and isc1Δ cells. Enzyme activity was detected as in (b). Values are means ± SD of three independent experiments. **p<0.01.

Fig. 7 — SIT4 disruption suppresses catalase A deficiency seen in isc1Δ cells and CTA1 overexpression partially suppresses the shortened chronological lifespan of isc1Δ cells. S. cerevisiae BY4741, isc1Δ, sit4Δ and sit4Δisc1Δ mutant cells were grown in YPD medium to post-diauxic phase (PDS). Catalaseactivity was determined (A) spectrophotometrically or (B) detected in situ after non-denaturing polyacrylamide gel electrophoresis, using the H₂O₂/peroxidase system. (C) S. cerevisiae BY4741 and isc1Δ mutant cells transformed with pPGK-M28-I (empty vector) or pCTA1-GFP were grown in SC medium lacking uracil to post-diauxic phase. Cells were washed twice with H₂O, and kept in H₂O at 26°C. The viability was determined by standard dilution plate counts and expressed as the percentage of the colony-forming units at time 0h. (D) CTA1 overexpression increases Cta1p activity in both S. cerevisiae BY4741 and isc1Δ cells. Enzyme activity was detected as in (b). Values are means ± SD of three independent experiments. **p<0.01.