Effects of pharmacological inhibitors on uptake (A), functional induction (B and C), and cellular distribution (D) of SSOs delivered to Hela/eGFP654 cells by L2K lipoplexes. C. After pre-incubation with methyl-β-CD for 1 hour, Hela/eGFP654 cells were treated with L2K/SSO-Tamra lipoplexes in the presence (Co-treated) or the absence (Pre-treated) of the inhibitor for 4 hours, the eGFP induction was measured by flow cytometry after 24 hours. In A–C, the results are normalized based on cells receiving no inhibitor (Control) as 100% and represent means and standard deviations of triplicate determinations.