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. 2011 Aug 1;138(15):3297–3306. doi: 10.1242/dev.058792

Fig. 3.

Fig. 3.

P. lividus endoderm gene expression was affected by Wnt6 perturbations, but mesoderm gene marker expression was not. Eggs were injected with dnWnt6 mRNA (0.2 μg/μl) or Wnt6 mRNA (0.5 μg/μl) and grown to the mesenchyme blastula stage. Expression of mesoderm and endoderm markers in the perturbed embryos was then compared with expression of those same markers in control mesenchyme blastulae. (A) Knockdown or ectopic expression of Wnt6 did not affect the expression of SkeT (a PMC marker), Delta, Gcm or Nlk (NSM markers that appear prior to mesenchyme blastula), or Papss (a nonskeletogenic marker that appears at mesenchyme blastula). (B) Knockdown of Wnt6 eliminated expression of the markers that normally are expressed in endoderm at mesenchyme blastula stage. Ectopic Wnt6 caused each of the same markers to be expressed throughout the animal hemisphere. Embryos are ∼90 μm in diameter.