Inhibition of vTT replication by Trpv1 agonists. (a) 7b-complementing cells were infected with vTT or vHG (control) virus and plated in culture medium containing increasing doses of capsaicin. Viral titers determined from supernatant medium at 72 h.p.i. are plotted against capsaicin concentration. Growth ratios, calculated as the ratio of vHG to vTT viral titers (p.f.u./ml) at each concentration of capsaicin are indicated at the top for each capsaicin concentration. Error bars, ± s.d., n = 4. (b) Complementing 7b cells infected with vTT or vHG (control) virus were plated in culture medium containing increasing doses of resiniferatoxin (0–1,000 nM final). Viral titers determined from supernatant samples are plotted against the resiniferatoxin concentration at 72 h post-infection. Growth ratios calculated as the ratio of vHG to vTT viral titers (p.f.u./ml) for each concentration of resiniferatoxin are indicated for each data point. Error bars, ± s.d.; n = 4.