Table II. Enrichment analysis of serodiagnostic antigens using computational predictions. A total of 115 serodiagnostic IgG and IgM antigens (126 exon hits) identified in this study were analyzed for enrichment against the full genome of 8,155 genes as described in the text. WoLF pSort was used to predict localizations. Other predictions were obtained from ToxoDB.org. Significant fold enrichment, or underrepresentation for certain features, are underlined and bold.

Predictions	Proteins in Category	Exons on Chip	Serodiagnostic
			Exon Hits	Gene Hits	FoldEnrich	p value
TMHMM = 0	6656	803	57	53	0.6	7.010E-18
TMHMM = 1	672	218	21	21	2.2	4.470E-04
TMHMM > 1	827	346	48	41	3.5	1.198E-13
TMHMM > = 5	341	215	37	31	6.4	2.125E-17
TMHMM > = 10	103	81	15	14	9.6	1.327E-10
Signal Peptide	1760	864	77	72	2.9	1.366E-21
No Signal Peptide	6395	503	49	43	0.5	1.366E-21
WoLF pSort Cytoskeleton	25	1	0	0	0.0	1.000E+00
WoLF pSort Cytosol	1402	120	13	11	0.6	4.306E-02
WoLF pSort E.R.	43	40	1	1	1.7	4.477E-01
WoLF pSort Extracellular	1213	230	26	24	1.4	6.008E-02
WoLF pSort Golgi	3	0	0	0	0.0	1.000E+00
WoLF pSort Mitochondria	842	202	11	11	1.0	1.000E+00
WoLF pSort Nuclear	3379	287	18	18	0.4	1.507E-08
WoLF pSort Peroxisome	26	7	1	1	2.8	3.013E-01
WoLF pSort Plasma membrane	1228	484	56	49	2.9	2.533E-13
WoLF pSort Null	321	17	1	1	0.2	1.347E-01
pl 0–5	1069	108	7	6	0.4	0.008
pl 5–7	2603	569	48	44	1.2	0.158
pl 7–9	2305	491	48	44	1.4	0.021
pl 9–14	2016	199	23	21	0.7	0.127
pl null	162	0	0	0	0.0	0.176
Ortholog group 4	6967	1353	121	113	1.2	6.036E-06
Other ortholog groups	1188	14	2	2	0.1	6.036E-06
Total Proteins	8155	1367	126	115