LRMH Serca2a staining results (A) relative to a negative stain (B) (lateral wedge only). The 16-replicate mean and standard deviation are shown for each depth of each heart, grouped by N-normal (green), d-DHF (black), and C-CRT (red). Four shapes within each state are used to represent each unique heart (filled square, filled triangle, open triangle, and open circle). The within-state means of the four hearts at each depth are shown by a horizontal line. A, shows the result of staining a LRMH array with a SERCA2a antibody, B, is an identical array that was used as a negative control (stained with secondary antibody only).