Table I. Variance components expressed as percent of total variability from nested ANOVA performed on RPMA Serca2a expression (negative control stain components).
| Variance components | Intensity | TPN adjusted | TPN and AMN adjusted | |||
|---|---|---|---|---|---|---|
| State (Norm,DHF,CRT) | 0.0 | (0.0) | 0.0 | (0.0) | 0.0 | (0.0) |
| Heart | 16.2 | (6.9) | 8.2 | (6.8) | 14 | (11.9) |
| Location (Ant,Lat) | 0.0 | (0.6) | 0.4 | (2.0) | 0.5 | (0.3) |
| Depth (Epi,Mid,Endo) | 9.4 | (0.0) | 38.9 | (0.0) | 75.2 | (2.6) |
| Section replicate | 17.5 | (28.3) | 1.6 | (0.0) | 3.1 | (3.3) |
| Lysate replicate | 19.4 | (50.8) | 0.0 | (24.0) | 1.8 | (15.8) |
| Within (unaccounted) | 37.6 | (13.3) | 50.9 | (67.3) | 5.4 | (66.0) |
| Biological | 25.6 | (7.5) | 47.5 | (8.8) | 89.7 | (14.8) |
| Nonbiological | 74.5 | (92.4) | 52.5 | (91.3) | 10.3 | (85.1) |