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SPR analysis of binding activity in culture media of two E. coli strains. Media cleared after growth of enterotoxigenic E. coli H10407 (A) and the nonpathogenic E. coli MG1655 (B) were injected over ganglioside GM1 nanodiscs captured on antibody coated sensorchips. The presented sensorgrams are produced by reference subtraction of the signal of binding to 900 RU GM1-free (control) nanodiscs from the signal of binding to 940 RU GM1 nanodiscs.
