Fig. 3.

SPR analysis of detergent based elution from nanodiscs captured by antibody. 6xHis-tagged Nanodiscs were injected over immobilized anti-tetra-His antibodies. Then the flow was changed to buffer without nanodiscs and subsequently an injection of detergent or buffer without detergent was injected (A). The remaining signal normalized to after detergent injection is reported in (B). The concentrations of detergent were as follows: Octyl glucopyranoside (OGP) 50 mm; cholate 25 mm; DHPC 5 mm; Triton X-100 (TX-100) 1 mm; P20 (tween 20) 0.1 mm.