Fig. 4.

Co-immunoprecipitation of proteins that bind to GM1 containing nanodiscs (A) and nanodiscs without GM1 (B). Fifteen microliters of the flow through, washes and eluents of 3xFLAG peptide incubation or DHPC incubation were subjected to SDS-PAGE as indicated. An aliquot of the FLAG M2 antibody that was used for immunoprecipitation is shown in (C) for comparison. The gel bands of the eluents are indicated with numbers that are also used in the text and Table I.