Figure 2.

Irradiation causes long-term defects in p53^7KR hematopoietic progenitor cells. (A) White blood cells (WBC) red blood cells (RBC), and and platelets (PLT) in WT, p53^7KR, and p53-null mice prior to irradiation. WT and 7KR: n = 13; null: n = 6. (B) Blood cell counts at 2 wk after 5 Gy of whole-body irradiation. (C) The fold changes of blood cell counts in p53^7KR mice related to WT mice 2 wk post-irradiation. (D) Mononucleated cell (MNCs) count in BM at baseline and 4, 14, and 28 d post-irradiation revealed insufficient recovery of BM cells in irradiated p53^7KR mice. (E) BM cells were isolated from nonirradiated mice and seeded for granulocytic/macrophage (CFU-GM), erythroid (BFU-E), and megakaryocytic (CFU-MK) progenitor cell colony-forming assay. (F) BM cells were immediately isolated from animals that were exposed to irradiation for progenitor colony-forming assay. Note: No colonies were found when 5 × 10⁶ p53^7KR BM cells were seeded for BFU-E assay. The error bars in D, E, and F represent SEM from three independent experiments.