Figure 3.
p537KR HSCs exhibit sensitivity to transplantation and to irradiation. BM from nonirradiated (A) and 4-d post-irradiated (B) WT or p537KR donor mice expressing the CD45.2 leukocyte cell surface marker were transplanted into lethally irradiated recipient mice in fixed ratios with WT competitor marrow cells expressing CD45.1. (C) p537KR HSCs did not compete as well as WT for long-term repopulation under both nonirradiated and irradiated conditions. At 6 wk and 12 wk post-transplantation, peripheral blood was withdrawn from the recipients and analyzed for the percentage of CD45.2 cells in total leukocytes. Results show mean ± SD from at least five animals. a, b: P < 0.05. c, d: P < 0.001.