Figure 4.
The hematopoietic defects in p537KR mice reside in HSCs/progenitor cells and not in the stem cell niche. (A) BM cells were isolated from nonirradiated mice and stained with antibodies against hematopoietic lineage (Lin) markers and cell surface marks Sca1 and c-Kit and analyzed by flow cytometry. Lin−Sca1+c-Kit+ subpopulation were gated as HSC-enriched LSK cells. Cell plots are shown in Supplemental Figure S4. (B) Antibody cocktail lineage markers CD41, CD48, and CD150 were used for the analysis. The Lin−CD41−CD48−CD150+ subpopulation was gated as HSC-enriched SLAM cells. Cell plots are shown in Supplemental Figure S4. (C) Introducing WT HSCs into p537KR mice by BM transplantation overcame the radiosensitivity of the animals. BM cells expressing CD45.1 were transplanted into lethally irradiated WT or p537KR mice. Twelve weeks later, the chimeric mice were exposed to 5 Gy of irradiation followed by CBC analysis. (A–C) Error bars represent the SEM from three animals.