Table 2.
Primers used in this study.
| Primer name | Primer sequence |
|---|---|
| csgE KO F no FRT | AGCGGTTTCCTGGGCAAACGATAACCTCAGGCGATAAAGCCACGCTGCCGCAAGCACT C |
| csgF KO R no FRT | ATAAGCGCTGCATGATTATTTTCCTTATGAAGCTGGGGAATAGGAACTTCAAGATCC |
| csgA F Kpn-RBS-Nco | GTTTGGTACCACACAGGAAACAGACCATGGCGAAACTTTTAAAAGTAGCAGCAATTGC AGC |
| csgA-cpxP R | CGCCTGAACCGACTTCAGCATTTGGGCCGCTATTATTACC |
| csgA-cpxP F | GGTAATAATAGCGGCCCAAATGCTGAAGTCGGTTCAGGCG |
| cpxP his R Pst (LR) | GTTTCTGCAGTTAGTGGTGGTGGTGGTGGTGCTGGGAACGTGAGTTGCTAC |
| csgE F Kpn-RBS-Nco | GTTTGGTACCACACAGGAAACAGACCATGGCGAAACGTTATTTACGCTGGATTGTGGC |
| csgE R 3Xs-Pst | GTTTCTGCAGTTATTAGATCCTTAGAATTCATCATGCGCCAAATCGCCC |
| csgE R BamHI | GTTTGGATCCTTAGAATTcATCATGCGCCAAATCGCC |
| csgF F Nco | GTTTCCATGGCACGTGTCAAACATGCAGTAG |
| csgF R Pst | GTTTCTGCAGTTAAAAATCGGTTGAGTTATTTTGTAAACC |
| a22cpxP F delA22 | CGGTAGCGCTCTGGCAGCTGAAGTCGGTTC |
| a22cpxP R delA22 | GAACCGACTTCAGCTGCCAGAGCGCTACCG |
| papDss(ala) KpnI mcpxP F NcoI |
CGAGCCATGGCGATTCGAAAAAAGATTCTGATGGCTGCCATCCCCCTGTTTGTTATATC CGGGGCAGACGCTGGTACCGCTGAAGTCGGTTCAGGCGATAAC |
| cpxP his R PstI (AN) | CTCGCTGCAGTTAGTGGTGGTGGTGGTGGTGCTGGGAACGTGAGTTGCTACTACT |
| papDss(ala) mcsgA F NcoI |
CGAGCCATGGCGATTCGAAAAAAGATTCTGATGGCTGCCATCCCCCTGTTTGTTATATC CGGGGCAGACGCTGGTGTTGTTCCTCAGTACGGCGG |
| csgA22 R KpnI | CGAGGGTACCATTTGGGCCGCTATTATTACCGCCACC |
| papD2 F KpnI | CGACGGTACCGAAGTATGGCAGGACCAGTTAATTCTG |
| papD2-his R PstI | CGACCTGCAGTTAGTGGTGGTGGTGGTGGTGTTTCTCTTTTTTCACAGAGCAACGGCTA C |
| Sec- CsgE For NcoI | GCGTTTCCATGGCCGTTGAGGTAGAAGTCCCGGGA |
| CsgE 6x His Rev BamHI |
GTTTAAAGCTTGGATCCTTAGTGATGGTGATGGTGATGGAATTCATCATGCGCCAAATC |