Table 3.
Antibodies used in this study.
Antibodies | Description | Dilution used in Western blot |
Source/Reference |
---|---|---|---|
α-CsgA | Polyclonal rabbit antiserum to CsgA | 1:5000-1:10,000 | (Barnhart et al., 2006) |
α CsgB | Polyclonal rabbit antiserum to the peptide (EGSSNRAKIDQTGDY) of CsgB |
1:2000 | (Robinson et al., 2006) |
α-CsgF | Polyclonal rabbit antiserum to CsgF | 1:5000-1:10,000 | (Nenninger et al., 2009) |
α-CsgG | Polyclonal rabbit antiserum to CsgG | 1:5000 | (Robinson et al., 2006) |
α-DsbA | Polyclonal rabbit antiserum to DsbA | 1:20,000 | Kind gift of the James Bardwell Laboratory, University of Michigan |
α-PapD | Polyclonal rabbit antiserum to PapD | 1:5000 | This study |
α-CT-His | Mouse anti- C-terminal his tag | 1:5000 | Invitrogen |
α CpxP | Polyclonal rabbit antiserum to CpxP | 1:10,000 | Kind gift of the Tracy Raivio Laboratory, University of Alberta |