Table 1.

Kinetic, thermodynamic, and spectroscopic parameters characterizing the binding of ASFV pol X to the ssDNA oligomers differing by the number of nucleotides, in buffer C (pH 7, 10 °C), containing 50 mM NaCl

ssDNA N	KN* (M−1)	K1	K2	k2 (s−1)	k−2 (s−1)	ΔF*	F2**	F3**	ΔH1 kcal/mol	ΔS1 cal/mol deg	ΔH2 kcal/mol	ΔS2 cal/ mol deg
10	(5.8 ± 0.9) × 105	2.0 ± 0.7) × 105	1.9 ± 0.6	155 ± 31	83 ± 16	0.8 ± 0.05	2.30 ± 0.02	1.50 ± 0.05	2.6 ± 0.8	33.3 ± 11	−0.13 ± 0.10	1.7 ± 1.1
14	(1.4 ± 0.3) × 106	5.4 ± 1.8) × 105	1.6 ± 0.5	135 ± 27	84 ± 15	0.7 ± 0.05	1.9 ± 0.05	1.6 ± 0.05	8.6 ± 2.8	59 ± 19	0.10 ± 0.10	1.3 ± 0.6
16	(1.8 ± 0.3) 106	7.2 ± 1.4) × 105	1.1 ± 0.3	105 ± 21	95 ± 19	0.9 ± 0.05	2.1 ± 0.05	1.7 ± 0.05	8.3 ± 2.8	56 ± 18	0.10 ± 0.10	0.5 ± 0.3
20	(4.4 ± 1.1) × 106	(2.5 ± 0.8) × 106	0.8 ± 0.3	80 ± 16	105 ± 21	0.85 ± 0.05	1.78 ± 0.05	1.94 ± 0.05	14.4 ± 4.8	80 ± 27	0.24 ± 0.10	0.40 ± 0.20

^*Determined in independent fluorescence titrations.

^**Values relative to the fluorescence, F₀ = 1 of the free DNA oligomers (details in text).