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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Protein Expr Purif. 2011 Mar 29;79(1):66–71. doi: 10.1016/j.pep.2011.03.012

Figure 1. Schematic diagram of the single fusion protein human sFcRn for expression in mammalian cells.

Figure 1

A, The B2M extracellular domain minus leader peptide is represented by the black bar. The stitched bar stands for the extracellular domain of the heavy chain. The two domains are connected with 3 copies of linker GGGGS sequence. The whole insert was flanked with BamHI and NotI restriction sites, which were used to subclone into pSecTag2A expression vector.

B, Primers for cloning human sFcRn as a single fusion protein into pSecTag2A. Primers B2Mrev and HCEfor have 12 nucleotides complementary to each other, through which the two PCR products were connected in an overlapping PCR. The sequences for restriction sites are underlined.