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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Biomaterials. 2011 Jun 12;32(27):6478–6486. doi: 10.1016/j.biomaterials.2011.05.045

Figure 1. LRAP stimulates osteogenesis of ST2 cells.

Figure 1

ST2 cells were subject to osteogenic induction in the presence or absence of LRAP (10 ng/ml). One week after osteogenic induction, cells were collected for analysis of bone marker protein. (A) Western blot analysis of Runt-related transcription factor 2 (Runx2). “OM”: osteogenic medium alone; “OM+LRAP”: osteogenic medium with LRAP (10 ng/ml). (B) Western blot analysis of osteocalcin (OCN). “OM”: osteogenic medium alone; “OM+LRAP”: osteogenic medium with LRAP (10 ng/ml). GAPDH was used as an internal control. Band intensity ratio was calculated by normalizing the band intensity for each sample with the band intensity of GAPDH. (C) Mineral deposition assayed with Alizarin Red staining. (D) Quantification of mineral deposition. The graphs represent mean±SD (a: P<0.01).