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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Biomaterials. 2011 Jun 12;32(27):6478–6486. doi: 10.1016/j.biomaterials.2011.05.045

Figure 5. LRAP treatment results in the upregulation of Wnt10b expression.

Figure 5

(A) ST2 cells were treated with LRAP (10 ng/ml) for various time periods and Wnt10b mRNA level was then measured with quantitative Real-time RT-PCR and normalized to GAPDH. “Control”: untreated; “LRAP”: treated with 10 ng/ml of LRAP. (B) ST2 cells were treated with LRAP (10 ng/ml) for various time periods. The conditioned medium from each time point was then collected and transferred to MC3T3 cells that had been transiently transfected with TOPFLASH or FOPFLASH reporter. Luciferase activity was measured using the Dual-Light reporter gene assay system (Applied Biosystems) 24 hours later. Relative luciferase activity was calculated by normalization of the average luciferase activity to the β-galactosidase activity. “Control FOPFLASH”: FOPFLASH-transfected MC3T3 cells with conditioned medium from untreated ST2 cells; “LRAP FOPFLASH”: FOPFLASH-transfected MC3T3 cells with conditioned medium from LRAP-treated ST2 cells; “Control TOPFLASH”: TOPFLASH-transfected MC3T3 cells with conditioned medium from untreated ST2 cells; “LRAP TOPFLASH”: TOPFLASH-transfected MC3T3 cells with conditioned medium from LRAP-treated ST2 cells. The graphs represent mean±SD (a: P<0.01).