Skip to main content
. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Biomaterials. 2011 Jun 12;32(27):6478–6486. doi: 10.1016/j.biomaterials.2011.05.045

Figure 6. Knockdown of Wnt10b expression by siRNA abolishes the effect of LRAP on the stimulation of osteogenesis and the inhibition of adipogenesis of ST2 cells.

Figure 6

ST2 cells were transfected with Wnt10b-specific siRNA for 48 hours and subsequently induced to osteogenic and adipogenic differentiation, respectively. (A) Two days after osteo-induction, RNA was isolated for quantitative Real-time RT-PCR analysis of osteoblast marker genes: Runx2, Osx, Dlx5 and type I collagen (coll. I). “OM”: osteo-induction in the absence of LRAP; “OM+LRAP”: osteo-induction in the presence of LRAP; “OM+siRNA”: osteo-induction in the presence of siRNA; “OM+LRAP+siRNA”: osteo-induction in the presence of LRAP and siRNA. (B) Two days after adipo-induction, RNA was isolated for quantitative Real-time RT-PCR analysis of adipocyte marker genes: C/EBPα and PPARγ “AM”: adipo-induction in the absence of LRAP; “AM+LRAP”: adipo-induction in the presence of LRAP; “AM+siRNA”: adipo-induction in the presence of siRNA; “AM+LRAP+siRNA”: adipo-induction in the presence of LRAP and siRNA. The graphs represent mean±SD (a: P<0.01).