ST2 cells were transfected with Wnt10b-specific siRNA for 48 hours and subsequently induced to osteogenic and adipogenic differentiation, respectively. (A) Two days after osteo-induction, RNA was isolated for quantitative Real-time RT-PCR analysis of osteoblast marker genes: Runx2, Osx, Dlx5 and type I collagen (coll. I). “OM”: osteo-induction in the absence of LRAP; “OM+LRAP”: osteo-induction in the presence of LRAP; “OM+siRNA”: osteo-induction in the presence of siRNA; “OM+LRAP+siRNA”: osteo-induction in the presence of LRAP and siRNA. (B) Two days after adipo-induction, RNA was isolated for quantitative Real-time RT-PCR analysis of adipocyte marker genes: C/EBPα and PPARγ “AM”: adipo-induction in the absence of LRAP; “AM+LRAP”: adipo-induction in the presence of LRAP; “AM+siRNA”: adipo-induction in the presence of siRNA; “AM+LRAP+siRNA”: adipo-induction in the presence of LRAP and siRNA. The graphs represent mean±SD (a: P<0.01).