. Author manuscript; available in PMC: 2012 Sep 1.

Published in final edited form as: Protein Expr Purif. 2011 May 27;79(1):16–24. doi: 10.1016/j.pep.2011.05.012

Table 1.

Yield of Aβ(1–40) from E. coli cell (~10 g) in 1 L of TB media at each purification step

	V (ml)	GST-Aβ(1–40) (mg)	Aβ(1–40) (mg)	Recovery (%)
Supernatant	60	ND	ND	ND
GSH column	100	95	13.7^a	100
Factor Xa cleavage	50	ND	13.7 ^a	ND
HPLC	50	ND	7.0	51

the estimated amount from the amount of fusion protein.