. Author manuscript; available in PMC: 2012 Sep 1.

Published in final edited form as: Protein Expr Purif. 2011 May 27;79(1):16–24. doi: 10.1016/j.pep.2011.05.012

Table 2.

Yield of Aβ(1–40) from E. coli cell (~3 g) in 1 L of M9 media for expression of an isotope labeled sample at each purification step

	V (ml)	GST-Ab1–40 (mg)	Aβ(1–40) (mg)	Recovery (%)
Supernatant	20	ND	ND	ND
GSH column	25	24	3.4 ^a	100
Factor Xa cleavage	10	ND	3.4 ^a	ND
HPLC	10	ND	1.5	44

the estimated amount from the amount of fusion protein.