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. Author manuscript; available in PMC: 2012 Jul 29.
Published in final edited form as: J Immunol Methods. 2011 Jun 13;370(1-2):75–85. doi: 10.1016/j.jim.2011.05.010

Fig 3.

Fig 3

Recognition of biotinylated gp120 and gp140 by ELISA and binding to macaque B cell lines. (a) SIV gp120 and gp140 were detected with monoclonal antibodies (MAbs) 3.11H, 3.4E, 1.9C and 1.10A (competition group IV, V, VI and VII, respectively) and plasmas (Plasma/SIV+) from sterilely protected SIVmac239Δnef immunized macaque (Mm376-04) followed by HRP-conjugated anti-human IgGs. ELISA plates were coated with biotinylated gp120 and gp140 or non-biotinylated gp120 and gp140. OD, optical density. Background values were obtained using control wells (no antigen). Bars represent OD value obtained after background subtraction. Error bars are based on standard deviation of OD values for triplicate wells for each antigen. (b) Anti-SIV ENV gp120-directed monoclonal antibody (MAb) producing B cell lines (3.11H and 3.4E) were stained with biotinylated gp120 and gp140 or buffer only. Cells were washed and further stained with APC-conjugated streptavidin. The control B cell line was developed from a naïve (SIV-negative) macaque.